This gene encodes a member of the Armadillo protein family, which function in adhesion between cells and signal transduction. Multiple translation initiation codons and alternative splicing result in many different isoforms being translated. Not all of the full-length natures of the described transcript variants have been determined. Read-through transcription also exists between this gene and the neighboring upstream thioredoxin-related transmembrane protein 2 (TMX2) gene.

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Immunocytochemical staining of HeLa cells using Catenin delta 1 antibody , Top panel: wild-type (WT); Bottom panal: Catenin delta 1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Catenin delta 1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

Validation of Catenin delta 1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Catenin delta 1 antibody and analyzed using BD flow cytometer.

Western blotting analysis using catenin delta 1 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with catenin delta 1 antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit.

Western blotting analysis using catenin delta 1 antibody . Catenin delta 1 expression in wild type (WT) and catenin delta 1 (CTNND1) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with catenin delta 1 antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

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