Enables ATP binding activity and magnesium ion binding activity. Involved in eukaryotic translation initiation factor 2 complex assembly. Located in cytoplasm.

推荐稀释比 WB: 1/2500-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Validation of Cell division cycle 123 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Cell division cycle 123 antibody and analyzed using BD flow cytometer.

Flow cytometric analysis of cell division cycle 123 expression in HepG2 cells using cell division cycle 123 antibody . Green, isotype control; red, cell division cycle 123.

Immunocytochemical staining of HepG2 cells with Cell division cycle 123 antibody . Nuclei were stained blue with DAPI; Cell division cycle 123 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.

Western blotting analysis using cell division cycle 123 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with cell division cycle 123 antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Western blotting analysis using cell division cycle 123 antibody . Cell division cycle 123 expression in wild type (WT) and cell division cycle 123 (CDC123) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with cell division cycle 123 antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

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