This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. This enzyme is a homodimeric flavoprotein. It is a central enzyme of cellular antioxidant defense, and reduces oxidized glutathione disulfide (GSSG) to the sulfhydryl form GSH, which is an important cellular antioxidant. Rare mutations in this gene result in hereditary glutathione reductase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been found.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Western blotting analysis using Glutathione-disulfide reductase antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Glutathione-disulfide reductase antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using Glutathione-disulfide reductase antibody . Glutathione-disulfide reductase expression in wild type (WT) and glutathione-disulfide reductase shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Glutathione-disulfide reductase antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Flow cytometric analysis of Glutathione-disulfide reductase expression in HeLa cells using Glutathione-disulfide reductase antibody . Green, isotype control; red, Glutathione-disulfide reductase.

Immunocytochemical staining of Hela cells with Glutathione-disulfide reductase antibody . Nuclei were stained blue with DAPI; Glutathione-disulfide reductase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Very low. Scale bar, 20 μm.

Immunocytochemical staining of HeLa cells using Glutathione-disulfide reductase antibody , Top panel: wild-type (WT); Bottom panal: Glutathione-disulfide reductase shRNA knockdown (KD). Nuclei were stained blue with DAPI; Glutathione-disulfide reductase was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.

Validation of Glutathione-disulfide reductase knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Glutathione-disulfide reductase antibody and analyzed using CytoFLEX.

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