The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different growth hormones. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. This gene was found to fuse to retinoic acid receptor-alpha (RARA) gene in a small subset of acute promyelocytic leukemias (APLL). The dysregulation of the signaling pathways mediated by this protein may be the cause of the APLL.

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Flow cytometric analysis of STAT5B expression in HepG2 cells using STAT5B antibody . Green, isotype control; red, STAT5B.

Western blotting analysis using STAT5B antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with STAT5B antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using STAT5B antibody . STAT5B expression in wild type (WT) and STAT5B shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with STAT5B antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Validation of STAT5B knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with STAT5B antibody and analyzed using BD flow cytometer.

Immunocytochemical staining of Hela cells using STAT5B antibody , Top panel: wild-type (WT); Bottom panal: STAT5B shRNA knockdown (KD). Nuclei were stained blue with DAPI; STAT5B was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

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