Involved in positive regulation of extrinsic apoptotic signaling pathway and regulation of wound healing. Predicted to be located in cell surface and ruffle. Predicted to be active in plasma membrane.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Flow cytometric analysis of TNF receptor superfamily member 12A expression in HT-1080 cells using TNF receptor superfamily member 12A antibody . Green, isotype control; red, TNF receptor superfamily member 12A.

Immunocytochemical staining of HT-1080 cells with TNF receptor superfamily member 12A antibody . Nuclei were stained blue with DAPI; TNF receptor superfamily member 12A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using TNF receptor superfamily member 12A antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TNF receptor superfamily member 12A antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using TNF receptor superfamily member 12A antibody . TNF receptor superfamily member 12A expression in wild type (WT) and TNF receptor superfamily member 12A shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with TNF receptor superfamily member 12A antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Validation of TNF receptor superfamily member 12A knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with TNF receptor superfamily member 12A antibody and analyzed using BD flow cytometer.

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