Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding the same protein, have been characterized.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Immunocytochemical staining of HeLa cells using ATG4C antibody , Top panel: wild-type (WT); Bottom panal: ATG4C shRNA knockdown (KD). Nuclei were stained blue with DAPI; ATG4C was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

Flow cytometric analysis of ATG4C expression in HeLa cells using ATG4C antibody (Cat #63403, 1:2,000). Green, isotype control; red, ATG4C.

Western blotting analysis using ATG4C antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ATG4C antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using ATG4C antibody . ATG4C expression in wild-type (WT) and ATG4C shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ATG4C antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit.

Validation of ATG4C knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with ATG4C antibody and analyzed using BD flow cytometer.

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