The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes an additional conjugation factor, E4, which is involved in multiubiquitin chain assembly. This gene is also the strongest candidate in the neuroblastoma tumor suppressor genes. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Validation of Ubiquitination factor E4 B knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Ubiquitination factor E4 B antibody and analyzed using BD flow cytometer.

Immunocytochemical staining of HeLa cells using Ubiquitination factor E4 B antibody , Top panel: wild-type (WT); Bottom panal: Ubiquitination factor E4 B shRNA knockdown (KD). Nuclei were stained blue with DAPI; Ubiquitination factor E4 B was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

Flow cytometric analysis of Ubiquitination factor E4 B expression in HepG2 cells using Ubiquitination factor E4 B antibody . Green, isotype control; red, Ubiquitination factor E4 B.

Immunocytochemical staining of HepG2 cells with Ubiquitination factor E4 B antibody . Nuclei were stained blue with DAPI; Ubiquitination factor E4 B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using Ubiquitination factor E4 B antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Ubiquitination factor E4 B antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using Ubiquitination factor E4 B antibody . Ubiquitination factor E4 B expression in wild type (WT) and Ubiquitination factor E4 B shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Ubiquitination factor E4 B antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

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