The product of this gene is a transcription factor that is rapidly induced after temperature stress and binds heat shock promoter elements (HSE). This protein plays a role in the regulation of lifespan. Expression of this gene is repressed by phosphorylation, which promotes binding by heat shock protein 90.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Flow cytometric analysis of Phospho-HSF1 (Ser303/Ser307) expression in HepG2 cells using Phospho-HSF1 (Ser303/Ser307) antibody . Green, isotype control; red,Phospho-HSF1 (Ser303/Ser307).

Immunocytochemical staining of HepG2 cells with phospho-HSF1 (Ser303/Ser307) antibody . Nuclei were stained blue with DAPI; Phospho-HSF1 (Ser303/Ser307) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using phospho-HSF1 (Ser303/Ser307) antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-HSF1 (Ser303/Ser307) antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Western blotting analysis using phospho-HSF1 (Ser303/Ser307) antibody . Phospho-HSF1 (Ser303/Ser307) expression in wild type (WT) and HSF1 shRNA knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with phospho-HSF1 (Ser303/Ser307) antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit . HSF1, heat shock transcription factor 1.

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