There are several documented fusion oncoproteins encoded partially by this gene. This gene also participates in several oncogenic rearrangements resulting in anaplastic lymphoma and mixoid chondrosarcoma, and may play a role in the NF-kappaB pathway. Multiple transcript variants have been found for this gene.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Validation of TFG knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with TFG antibody and analyzed using BD flow cytometer.

Flow cytometric analysis of TFG expression in HepG2 cells using TFG antibody . Green, isotype control; red, TFG.

Immunocytochemical staining of HepG2 cells with TFG antibody . Nuclei were stained blue with DAPI; TFG  was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Western blotting analysis using TFG antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TFG antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using TFG antibody . TFG expression in wild type (WT) and TFG shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with TFG antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Immunocytochemical staining of HeLa cells using TFG antibody , Top panel: wild-type (WT); Bottom panal: TFG shRNA knockdown (KD). Nuclei were stained blue with DAPI; TFG was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

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