The protein encoded by this gene is a member of the CMGC family of serine/threonine protein kinases. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. Altered expression of this gene has been observed in multiple human cancers. A mutation in this gene resulting in reduced cell proliferation, and impaired cell motility and polarity, and has been identified in patients with primary microcephaly.

推荐稀释比 WB: 1/4000-1/20000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Western blotting analysis using Cyclin dependent kinase 6 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Cyclin dependent kinase 6 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Western blotting analysis using cyclin dependent kinase 6 antibody . Cyclin dependent kinase 6 expression in wild-type (WT) and cyclin dependent kinase 6 (CDK6) knockout (KO) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with cyclin dependent kinase 6 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Flow cytometric analysis of Cyclin dependent kinase 6 expression in HAP-1 cells using Cyclin dependent kinase 6 antibody . Green, isotype control; red, Cyclin dependent kinase 6.

Immunocytochemical staining of HAP-1 cells with cyclin dependent kinase 6 antibody . Nuclei were stained blue with DAPI; Cyclin dependent kinase 6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

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