Enables GTPase binding activity; T cell receptor binding activity; and cardiolipin binding activity. Involved in intracellular calcium ion homeostasis; mitochondrion organization; and protein complex oligomerization. Acts upstream of or within T cell receptor signaling pathway. Located in several cellular components, including immunological synapse; membrane raft; and mitochondrial envelope.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Western blotting analysis using Stomatin like 2 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Stomatin like 2 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using Stomatin like 2 antibody . Stomatin like 2 expression in wild type (WT) and Stomatin like 2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Stomatin like 2 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Validation of Stomatin like 2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Stomatin like 2 antibody and analyzed using BD flow cytometer.

Flow cytometric analysis of Stomatin like 2 expression in HepG2 cells using Stomatin like 2 antibody . Green, isotype control; red, Stomatin like 2.

Immunocytochemical staining of HepG2 cells with Stomatin like 2 antibody . Nuclei were stained blue with DAPI; Stomatin like 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Immunocytochemical staining of HeLa cells using Stomatin like 2 antibody , Top panel: wild-type (WT); Bottom panal: Stomatin like 2 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Stomatin like 2 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

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