This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. The encoded protein is necessary for p53-mediated caspase activation and apoptosis. Mutations in this gene are a cause of Charcot-Marie-Tooth disease type 4D, and expression of this gene may be a prognostic indicator for several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

本产品仅用作科学研究！

Immunocytochemical staining of HeLa cells using Phospho-NDRG1 (Ser330) antibody , Top panel: wild-type (WT); Bottom panal: Phospho-NDRG1 (Ser330) shRNA knockdown (KD). Nuclei were stained blue with DAPI; Phospho-NDRG1 (Ser330) was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

Flow cytometric analysis of phospho-NDRG1 (Ser330) expression in HepG2 cells using phospho-NDRG1 (Ser330) antibody . Green, isotype control; red, phospho-NDRG1 (Ser330).

Immunocytochemical staining of HepG2 cells with Phospho-NDRG1 (Ser330) antibody . Nuclei were stained blue with DAPI; Phospho-NDRG1 (Ser330) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using phospho-NDRG1 (Ser330) antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-NDRG1 (Ser330) antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Western blotting analysis using phospho-NDRG1 (Ser330) antibody . Phospho-NDRG1 (Ser330) expression in wild-type (WT) and NDRG1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with phospho-NDRG1 (Ser330) antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit . NDRG1, N-myc downstream regulated 1.

Validation of NDRG1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Phospho-NDRG1 (Ser330) antibody and analyzed using BD flow cytometer.

Store at -20℃ for one year.