This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating their deactivation. Several transcript variants encoding different isoforms have been described for this gene.

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Flow cytometric analysis of G protein-coupled receptor kinase 6 expression in HepG2 cells using G protein-coupled receptor kinase 6 antibody . Green, isotype control; red, G protein-coupled receptor kinase 6.

Western blotting analysis using G protein-coupled receptor kinase 6 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with G protein-coupled receptor kinase 6 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Western blotting analysis using G protein-coupled receptor kinase 6 antibody . G protein-coupled receptor kinase 6 expression in wild type (WT) and G protein-coupled receptor kinase 6 (GRK6) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with G protein-coupled receptor kinase 6 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Validation of G protein-coupled receptor kinase 6 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with G protein-coupled receptor kinase 6 antibody and analyzed using BD flow cytometer.

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