This gene encodes a cytoplasmic enzyme in the trans-sulfuration pathway that converts cystathione derived from methionine into cysteine. Glutathione synthesis in the liver is dependent upon the availability of cysteine. Mutations in this gene cause cystathioninuria. Alternative splicing of this gene results in three transcript variants encoding different isoforms.

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Flow cytometric analysis of cystathionine gamma-lyase expression in HepG2 cells using cystathionine gamma-lyase antibody . Green, isotype control; red, Cystathionine Gamma-Lyase.

Western blotting analysis using cystathionine gamma-lyase antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with cystathionine gamma-lyase antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Western blotting analysis using cystathionine gamma-lyase antibody . Cystathionine gamma-lyase expression in wild type (WT) and cystathionine gamma-lyase (CTH) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with cystathionine gamma-lyase antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

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