This gene encodes a member of the retinoic acid (RA, a form of vitamin A) binding protein family and lipocalin/cytosolic fatty-acid binding protein family. The protein is a cytosol-to-nuclear shuttling protein, which facilitates RA binding to its cognate receptor complex and transfer to the nucleus. It is involved in the retinoid signaling pathway, and is associated with increased circulating low-density lipoprotein cholesterol. Alternatively spliced transcript variants encoding the same protein have been found for this gene.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

本产品仅用作科学研究！

Validation of CRABP2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with CRABP2 antibody and analyzed using BD flow cytometer.

Flow cytometric analysis of CRABP2 expression in C2C12 cells using CRABP2 antibody . Green, isotype control; red, CRABP2.

Immunocytochemical staining of C2C12 cells with CRABP2 antibody . Nuclei were stained blue with DAPI; CRABP2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: Medium. Scale bar, 20 μm.

Western blotting analysis using CRABP2 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CRABP2 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit . CRABP2, cellular retinoic acid binding protein 2.

Western blotting analysis using CRABP2 antibody . CRABP2 expression in wild-type (WT) and CRABP2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with CRABP2 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Store at -20℃ for one year.