The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Immunocytochemical staining of HepG2 cells with phospho-CDC6 (Ser106) antibody . Nuclei were stained blue with DAPI; Phospho-CDC6 (Ser106) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using phospho-CDC6 (Ser106) antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-CDC6 (Ser106) antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Western blotting analysis using phospho-CDC6 (Ser106) antibody . Phospho-CDC6 (Ser106) expression in wild type (WT) and CDC6 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with phospho-CDC6 (Ser106) antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit . CDC6, cell division cycle 6.

Validation of CDC6 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Phospho-CDC6 (Ser106) antibody and analyzed using BD flow cytometer.

Immunocytochemical staining of HeLa cells using Phospho-CDC6 (Ser106) antibody , Top panel: wild-type (WT); Bottom panal: Phospho-CDC6 (Ser106) shRNA knockdown (KD). Nuclei were stained blue with DAPI; Phospho-CDC6 (Ser106) was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.

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