The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein has been shown to possess PPIase activity and, similar to other family members, can bind to the immunosuppressant cyclosporin A.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Validation of Peptidylprolyl isomerase D knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Peptidylprolyl isomerase D antibody and analyzed using BD flow cytometer.

Flow cytometric analysis of Peptidylprolyl isomerase D expression in HepG2 cells using Peptidylprolyl isomerase D antibody . Green, isotype control; red, Peptidylprolyl isomerase D.

Immunocytochemical staining of HepG2 cells with Peptidylprolyl isomerase D antibody . Nuclei were stained blue with DAPI; Peptidylprolyl isomerase D was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using Peptidylprolyl isomerase D antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Peptidylprolyl isomerase D antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using Peptidylprolyl isomerase D antibody . Peptidylprolyl isomerase D expression in wild type (WT) and Peptidylprolyl isomerase D shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Peptidylprolyl isomerase D antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Immunocytochemical staining of HeLa cells using Peptidylprolyl isomerase D antibody , Top panel: wild-type (WT); Bottom panal: Peptidylprolyl isomerase D shRNA knockdown (KD). Nuclei were stained blue with DAPI; Peptidylprolyl isomerase D was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.

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