This gene was identified by its translocation in a case of mucosa-associated lymphoid tissue (MALT) lymphoma. The protein encoded by this gene contains a caspase recruitment domain (CARD), and has been shown to induce apoptosis and to activate NF-kappaB. This protein is reported to interact with other CARD domain containing proteins including CARD9, 10, 11 and 14, which are thought to function as upstream regulators in NF-kappaB signaling. This protein is found to form a complex with MALT1, a protein encoded by another gene known to be translocated in MALT lymphoma. MALT1 and this protein are thought to synergize in the activation of NF-kappaB, and the deregulation of either of them may contribute to the same pathogenetic process that leads to the malignancy. Alternative splicing results in multiple transcript variants.

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Validation of bcl10 immune signaling adaptor knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with bcl10 immune signaling adaptor antibody and analyzed using BD flow cytometer.

Flow cytometric analysis of bcl10 immune signaling adaptor expression in HepG2 cells using BCL10 Immune Signaling Adaptor antibody . Green, isotype control; red, BCL10 Immune Signaling Adaptor.

Immunocytochemical staining of HepG2 cells with bcl10 immune signaling adaptor antibody . Nuclei were stained blue with DAPI; Bcl10 immune signaling adaptor was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using bcl10 immune signaling adaptor antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with bcl10 immune signaling adaptor antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Western blotting analysis using bcl10 immune signaling adaptor antibody . Bcl10 immune signaling adaptor expression in wild type (WT) and bcl10 immune signaling adaptor (BCL10) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with bcl10 immune signaling adaptor antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

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