Alzheimer's disease (AD) patients with an inherited form of the disease carry mutations in the presenilin proteins (PSEN1; PSEN2) or in the amyloid precursor protein (APP). These disease-linked mutations result in increased production of the longer form of amyloid-beta (main component of amyloid deposits found in AD brains). Presenilins are postulated to regulate APP processing through their effects on gamma-secretase, an enzyme that cleaves APP. Also, it is thought that the presenilins are involved in the cleavage of the Notch receptor, such that they either directly regulate gamma-secretase activity or themselves are protease enzymes. Several alternatively spliced transcript variants encoding different isoforms have been identified for this gene, the full-length nature of only some have been determined.

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Flow cytometric analysis of Phospho-Presenilin 1 (Ser310) expression in HeLa cells using Phospho-Presenilin 1 (Ser310) antibody . Green, isotype control; red, Phospho-Presenilin 1 (Ser310).

Immunocytochemical staining of Hela cells with Phospho-Presenilin 1 (Ser310) antibody . Nuclei were stained blue with DAPI; Phospho-Presenilin 1 (Ser310) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium.Scale bar, 20 μm.

Validation of Presenilin 1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Phospho-Presenilin 1 (Ser310) antibody and analyzed using BD flow cytometer.

Western blotting analysis using phospho-presenilin 1 (Ser310) antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-presenilin 1 (Ser310) antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Western blotting analysis using phospho-presenilin 1 (Ser310) antibody . Phospho-presenilin 1 (Ser310) expression in wild-type (WT) and PSEN1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with phospho-presenilin 1 (Ser310) antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit . PSEN1, presenilin 1.

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