This gene encodes a member of the O class of winged helix/forkhead transcription factor family. Proteins encoded by this class are regulated by factors involved in growth and differentiation indicating they play a role in these processes. A translocation involving this gene on chromosome X and the homolog of the Drosophila trithorax gene, encoding a DNA binding protein, located on chromosome 11 is associated with leukemia. Multiple transcript variants encoding different isoforms have been found for this gene.

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Flow cytometric analysis of Forkhead box O4 expression in HeLa cells using Forkhead box O4 antibody . Green, isotype control; red, Forkhead box O4.

Immunocytochemical staining of HeLa cells with Forkhead box O4 antibody . Nuclei were stained blue with DAPI; Forkhead box O4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using Forkhead box O4 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Forkhead box O4 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using Forkhead box O4 antibody . Forkhead box O4 expression in wild type (WT) and Forkhead box O4 shRNA knockdown (KD) 293T cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Forkhead box O4 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Immunocytochemical staining of 293T cells using Forkhead box O4 antibody , Top panel: wild-type (WT); Bottom panal: Forkhead box O4 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Forkhead box O4 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

Western blotting analysis using Forkhead box O4 antibody (Cat.#62585).Forkhead box O4 expression in wild type (WT) and FOXO4 knockout (KO) 293T cells with 30 μg of Total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Forkhead box O4 antibody (Cat.#62585, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

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