The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Western blotting analysis using FGF2 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FGF2 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit . FGF2, fibroblast growth factor 2.

Western blotting analysis using fibroblast growth factor 2 antibody . Fibroblast growth factor 2 expression in wild-type (WT) and fibroblast growth factor 2 (FGF2) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with fibroblast growth factor 2 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Immunocytochemical staining of HeLa cells using FGF2 antibody , Top panel: wild-type (WT); Bottom panal: FGF2 shRNA knockdown (KD). Nuclei were stained blue with DAPI; FGF2 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

Flow cytometric analysis of FGF2 expression in HepG2 cells using FGF2 antibody . Green, isotype control; red, FGF2.

Immunocytochemical staining of HepG2 cells with FGF2 antibody . Nuclei were stained blue with DAPI; FGF2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Validation of FGF2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with FGF2 antibody and analyzed using BD flow cytometer.

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