The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor, thus inducing receptor dimerization and tyrosine autophosphorylation leading to cell proliferation. Mutations in this gene are associated with lung cancer. EGFR is a component of the cytokine storm which contributes to a severe form of Coronavirus Disease 2019 (COVID-19) resulting from infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000; IHC-P: 1/100-1/200

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Western blotting analysis using epidermal growth factor receptor antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with epidermal growth factor receptor antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Western blotting analysis using epidermal growth factor receptor antibody . Epidermal growth factor receptor expression in wild type (WT) and EGFR shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with epidermal growth factor receptor antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Flow cytometric analysis of epidermal growth factor receptor expression in HepG2 cells using epidermal growth factor receptor antibody . Green, isotype control; red, Epidermal growth factor receptor.

Validation of epidermal growth factor receptor knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HepG2 cells were stained with epidermal growth factor receptor antibody and analyzed using BD flow cytometer.

Immunocytochemical staining of HepG2 cells with epidermal growth factor receptor antibody . Nuclei were stained blue with DAPI; Epidermal growth factor receptor was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Immunocytochemical staining of HepG2 cells using epidermal growth factor receptor antibody , Top panel: wild-type (WT); Bottom panal: Adiponectin receptor 1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; epidermal growth factor receptor was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.

Immunohistochemistry was performed on paraffin-embedded human lung adenocarcinoma using epidermal growth factor receptor antibody . Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.

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