The protein encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This protein is considered a crucial component of an effective regenerative response in the nervous system. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

推荐稀释比 WB: 1/1000-1/50000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Flow cytometric analysis of Growth associated protein 43 expression in HAP-1 cells using Growth associated protein 43 antibody . Green, isotype control; red, Growth associated protein 43.

Immunocytochemical staining of HAP-1 cells with Growth associated protein 43 antibody . Nuclei were stained blue with DAPI; Growth associated protein 43 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using Growth associated protein 43 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Growth associated protein 43 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using Growth associated protein 43 antibody . Growth associated protein 43 expression in wild type (WT) and growth associated protein 43 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Growth associated protein 43 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Validation of Growth associated protein 43 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Growth associated protein 43 antibody and analyzed using CytoFLEX.

Immunocytochemical staining of HeLa cells using GAP43 antibody , Top panel: wild-type (WT); Bottom panal: GAP43 shRNA knockdown (KD). Nuclei were stained blue with DAPI; GAP43 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.

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