This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants.

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Flow cytometric analysis of DNA methyltransferase 1 expression in HepG2 cells using DNA methyltransferase 1 antibody . Green, isotype control; red, DNA methyltransferase 1.

Immunocytochemical staining of HepG2 cells with DNA methyltransferase 1 antibody . Nuclei were stained blue with DAPI; DNA methyltransferase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Western blotting analysis using DNA methyltransferase 1 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DNA methyltransferase 1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit.

Western blotting analysis using DNA methyltransferase 1 antibody . DNA methyltransferase 1 expression in wild type (WT) and DNA methyltransferase 1 (DNMT1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with DNA methyltransferase 1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Validation of DNA methyltransferase 1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with DNA methyltransferase 1 antibody and analyzed using BD flow cytometer.

Immunocytochemical staining of Hela cells using DNA methyltransferase 1 antibody , Top panel: wild-type (WT); Bottom panal: DNA methyltransferase 1 shRNA knockdown (KD). Nuclei were stained blue with DAPI;DNA methyltransferase 1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.

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