This gene encodes the beta-subunit of glucosidase II, an N-linked glycan-processing enzyme in the endoplasmic reticulum. The encoded protein is an acidic phosphoprotein known to be a substrate for protein kinase C. Mutations in this gene have been associated with the autosomal dominant polycystic liver disease. Alternative splicing results in multiple transcript variants.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Flow cytometric analysis of PRKCSH expression in Hela cells using PRKCSH antibody . Green, isotype control; red, PRKCSH.

Immunocytochemical staining of Hela cells with PRKCSH antibody . Nuclei were stained blue with DAPI; PRKCSH was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using PRKCSH antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PRKCSH antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using PRKCSH antibody and PRKCSH shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with PRKCSH antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Immunocytochemical staining of HeLa cells using PRKCSH antibody , Top panel: wild-type (WT); Bottom panal: PRKCSH shRNA knockdown (KD). Nuclei were stained blue with DAPI; PRKCSH was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.

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