This gene encodes an enzyme that catalyzes the NAD/NADH-dependent, reversible oxidation of malate to oxaloacetate in many metabolic pathways, including the citric acid cycle. Two main isozymes are known to exist in eukaryotic cells: one is found in the mitochondrial matrix and the other in the cytoplasm. This gene encodes the cytosolic isozyme, which plays a key role in the malate-aspartate shuttle that allows malate to pass through the mitochondrial membrane to be transformed into oxaloacetate for further cellular processes. Alternatively spliced transcript variants have been found for this gene. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Pseudogenes have been identified on chromosomes X and 6.

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Flow cytometric analysis of Malate dehydrogenase 1 expression in HepG2 cells using Malate dehydrogenase 1 antibody . Green, isotype control; red, Malate dehydrogenase 1.

Immunocytochemical staining of HepG2 cells with Malate dehydrogenase 1 antibody . Nuclei were stained blue with DAPI; Malate dehydrogenase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using Malate dehydrogenase 1 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Malate dehydrogenase 1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Western blotting analysis using malate dehydrogenase 1 antibody . Malate dehydrogenase 1 expression in wild-type (WT) and malate dehydrogenase 1 (MDH1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with malate dehydrogenase 1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Immunocytochemical staining of HeLa cells using Malate dehydrogenase 1 antibody , Top panel: wild-type (WT); Bottom panal: Malate dehydrogenase 1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Malate dehydrogenase 1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

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