Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473.

推荐稀释比 WB:1/2000-1/5000; IHC/1/50-1/100; ICC/IF:1/100-1/200; FC:1/20; IP:1/20-1/50

Immunofluorescence analysis of AKT1 in Hela using AKT1 antibody.

Western blot analysis of AKT1 in C2C12, THP-1, 3T3 and Jurkat lysates using AKT1 antibody.

Western blot analysis of AKT1 expression in HisAKT1 trans CHO-K1, HisAKT2 trans CHO-K1 and CHO-K1 lysates using AKT1 antibody

Immunohistochemistry analysis of paraffin-embedded Human colon using AKT1 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Western blot analysis of AKT1 in MCF-7 lysates using AKT1 antibody.

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.