Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21. 3.

推荐稀释比 WB:1/1000-1/2000; IF:1/50-1/200; ChIP:1/20

Western blot analysis of DiMethyl-Histone H3 (Lys9) in Hela lysates ，H3 protein using DiMethyl-Histone H3 (Lys9) antibody.

DiMethyl-Histone H3K9 pAb Dotblot analysis of all sorts of methylation peptides using DiMethyl-Histone H3K9 antibody.

Immunofluorescence analysis of DiMethyl-Histone H3 (Lys9) in 293T using DiMethyl-Histone H3K9 antibody,and DAPI (blue).

Chromatin Immunoprecipitation DiMethyl-Histone H3K9 pAb Chromatin immunoprecipitation analysis of extracts of 293 cell line, using DiMethyl-Histone H3K9 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the rat ios of the immunoprecipitated DNA to the input.

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