HiActi® Recombinant Human EGF Protein,hFc Tag
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产品介绍

The epidermal growth factor (EGF) family of peptides encodes several proteins that can function as growth factors . The EGF-like peptides, with the exception of proteins of the EGF-CFC subfamily, bind and activate tyrosine kinase receptors that belong to the erbB family.

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产品特色

高活性、高纯度、低内毒素、高批间一致性

应用案例




                                        SDS-PAGE


                     


Human EGF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.



                                                   HPLC


         


The purity of Human EGF is greater than 95% as determined by SEC-HPLC.



                                                   ELISA Data


         


Immobilized Biotinylated Human EGFR, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human EGF, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA.



                                                 SPR Data


         


Human EGFR, His Tag captured on CM5 Chip via anti-his antibody can bind Human EGF, hFc Tag with an affinity constant of 0.39 nM as determined in SPR assay.



                                         Cell Based Assay


         


Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblast cells. The ED50 for this effect is typically 0.2 - 1.5 ng/mL.

存储条件

-25~-15℃保存,收到货之日起,有效期1年。复溶后, -85~-65℃保存,3个月有效期。避免反复冻融。

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