Isocitrate dehydrogenases (IDH) catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Mutations affecting Arg-132 are tissue-specific, and suggest that this residue plays a unique role in the development of high-grade gliomas. Mutations of Arg-132 to Cys, His, Leu or Ser abolish magnesium binding and abolish the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R-2-hydroxyglutarate. Elevated levels of R-2-hydroxyglutarate are correlated with an elevated risk of malignant brain tumors.

推荐稀释比 ELISA:1/1000-1/5000;WB:1/100-1/1000;ICC/IF:1/50-1/100;IHC-P:1/50-1/100

Western blot analysis of recombinant IDH1(R132P) and wild type proteins. Purified His-tagged IDH1(R132P) (lane 1) and corresponding wild type IDH1 protein (lane 2) were blotted with Anti-IDH1(R132P) monoclonal antibody.

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