Isocitrate dehydrogenase (IDH) catalyzes the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Mutations of Arg-132 in IDH to Cys, His, Leu or Ser abolish magnesium binding and the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R-2-hydroxyglutarate. Elevated levels of R-2-hydroxyglutarate are correlated with an elevated risk of malignant brain tumors.

推荐稀释比 ELISA:1/1000-1/5000;WB:1/100-1/1000;ICC/IF:1/50-1/100;IHC-P:1/50-1/100

Western blot analysis of recombinant IDH1(R132H) and wild type proteins.Purified His-tagged IDH1(R132H) protein and wild type protein (amino acid 1-240) were blotted with anti-IDH1(R132H) mouse antibody (Left panel) or with anti-IDH1 mouse antibody (right panel).

-25 ~ -15℃保存，收到货之后有效期1年，避免反复冻融。