Receptor-interacting serine/threonine-protein kinase 1 is a serine-threonine kinase which transduces inflammatory and cell-death signals (programmed necrosis) following death receptors ligation, activation of pathogen recognition receptors (PRRs), and DNA damage. Upon activation of TNFR1 by the TNF-alpha family cytokines, TRADD and TRAF2 are recruited to the receptor. Phosphorylates DAB2IP at 'Ser-728' in a TNF-alpha-dependent manner, and thereby activates the MAP3K5-JNK apoptotic cascade. RIPK1 is phosphorylated at several sites within the kinase domain that are sensitive to Nec-1, including Ser14, Ser15, Ser161, and Ser166.

推荐稀释比 WB:1/500-1/1000;IHC:1/50-1/100;IF:1/50-1/200;IP:1/50-1/500

Western blot and IP analysis of 661W cells, treated with combinations of the following treatments as indicated: Z-VAD (50 μM, 2 hr), mTNF-α(50 ng/ml, 2 hr), SM-164 (100 nM, 2 hr) or necrostatin-1 (Nec-1s, 30 μM, 2 hr), using R10014 Antibody. Western blot analysis was performed using a RIPK1 mouse Ab.

-25 ~ -15℃保存，收到货之后有效期1年，避免反复冻融。