PDCD4 was a suppressor of neoplastic transformation. It interacts with eukaryotic initiation factors eIF4AI and eIF4AII. PDCD4 colocalizes with eIF4A in the cytoplasm and inhibits the activity of eIF4A as an ATP-dependent RNA helicase. PDCD4 inhibits cap-dependent translation. Upon stimulation by mitogens, PDCD4 is phosphorylated on Ser67 by S6K1 kinase and then degraded through the ubiquitin pathway leading to enhanced translation and cell growth.

推荐稀释比 WB:1/500-1/1000;IHC-P:1/50-1/100;ICC/IF:1/50

Western blot analysis of PDCD4 in lysates of HeLa,Jurkat and HEK-293T using PDCD4 antibody.

Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma using PDCD4 antibody.High pressure and temperature Tris/EDTA (PH 9.0) used for antigen retrieval.The brown color represents the positive signal observed with PDCD4 antibody.

Immunocytochemistry analysis of PDCD4(green) in HeLa cells using PDCD4 antibody,and DAPI(blue).

-25 ~ -15℃保存，收到货之后有效期1年，避免反复冻融。