eIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types.

推荐稀释比 WB:1/500-1/1000;IHC:1/100-1/200;IF:1/50-1/200;IP:1/50

Western blot analysis of eIF4E (phosphoS209) in (1) HEK293 lysates; (2) Mouse spleen lysates using Phospho-eIF4E (Ser209) antibody.

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Phospho-eIF4E (S209) antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

-25 ~ -15℃保存，收到货之后有效期1年，避免反复冻融。