Produces green light with a wavelength of 562 nm. Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.

Reactivity:Firefly

推荐稀释比 WB:1/500-1/1000;IF:1:50/1:200

Western blot analysis of Firefly Luciferase Antibody Nterminal in (1) Firefly Luciferase transfected 293T lysates; (2) HeLa lysates; (3) NIH/3T3 lysates; (4) C6 lysates using Firefly Luciferase antibody.

-25 ~ -15℃保存，收到货之后有效期1年，避免反复冻融。