EEA1 is an early endosomal marker and a Rab5 effector protein essential for early endosomal membrane fusion and trafficking (1-2). The carboxy terminus of EEA1 contains a FYVE domain which binds to phosphatidylinositol-3-phosphate (PtdIns(3)P), targeting EEA1 to early endosomes (3). The stable association of EEA1 with the endosomal membrane is regulated by PI3 kinase, Rab5 and calcium/calmodulin (4-6). Once on the membrane, EEA1 interacts with Rab5, NSF and syntaxin 13 to promote early endosomal membrane docking and fusion (7).

推荐稀释比 WB:1/500-1/1000;IF:1:50/1:200

Western blot analysis of EEA1 in NIH/3T3,C6,HeLa,Jurkat,JAR lysates using EEA1 antibody.

Western blot analysis of EEA1 in (1) HeLa lysates; (2) NIH/3T3 lysates using EEA1 antibody.

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