The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

推荐稀释比 WB:1/500-1/1000;IHC:1/50-1/100;IF:1/50-1/200;IP:1/20

Western blot analysis of p40phox in HL-60, U251 lysates using NCF4 antibody.

Immunohistochemistry analysis of paraffin-embedded Human tonsil using p40phox antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Immunocytochemistry analysis of p4phox (green) in hela using p4phox antibody,and DAPI(blue)

-25 ~ -15℃保存，收到货之后有效期1年，避免反复冻融。