Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation.

推荐稀释比 WB:1/500-1/1000;IP:1/20

Western blot analysis of APPBP1 in SKOV-3 lysates using APPBP1 antibody.

-25 ~ -15℃保存，收到货之后有效期1年，避免反复冻融。