Bifunctional DNA N-glycosylase with associated apurinic/apyrimidinic (AP) lyase function that catalyzes the first step in base excision repair (BER), the primary repair pathway for the repair of oxidative DNA damage. The DNA N-glycosylase activity releases the damaged DNA base from DNA by cleaving the N-glycosidic bond, leaving an AP site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination. Primarily recognizes and repairs oxidative base damage of pyrimidines. Has also 8-oxo-7,8-dihydroguanine (8-oxoG) DNA glycosylase activity. Acts preferentially on DNA damage opposite guanine residues in DNA. Is able to process lesions in nucleosomes without requiring or inducing nucleosome disruption.

推荐稀释比 WB:1/500-1/1000;IF:1:50/1:200

Immunocytochemistry analysis of NTH1 (green) in Hela using NTH1 antibody,and DAPI(blue).

Western blot analysis of NTH1 in K562, Hela lysates using NTH1 antibody.

-25 ~ -15℃保存，收到货之后有效期1年，避免反复冻融。