5T4 is a 72 kDa transmembrane glycoprotein that exhibits restricted expression in human and mouse adult tissues, is upregulated on many carcinomas and tumour expression correlates with poorer clinical outcome in ovarian, gastric and colorectal cancers. mES cells lack cell surface expression of the 5T4 antigen, and both protein and mRNA are rapidly upregulated following induction of differentiation. This proteins expression correlates with downregulation of OCT-4 and Tra-1–60.

推荐稀释比 WB:1/500-1/1000;IHC:1/50-1/100

Western blot analysis of 5T4 in Caco2 lysates using 5HT4 Receptor antibody.

Immunohistochemistry analysis of paraffin-embedded Human uters cancer using 5T4 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

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