HLA-DOB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DOA) and a beta chain (DOB), both anchored in the membrane. It is located in intracellular vesicles. DO suppresses peptide loading of MHC class II molecules by inhibiting HLA-DM. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail.

推荐稀释比 WB:1/500-1/1000;IHC:1/50-1/100

Western blot analysis of HLADOB in HepG2, Hela, 293T lysates using HLADOB antibody.

Immunohistochemistry analysis of paraffin-embedded Human prostate cancer using HLA DOB antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

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