Host cell factor (HCF) is a cellular cofactor required for the activation of VP16 which expresses the herpes simplex virus immediate early gene. V16 binds to HCF through a 4-amino acid motif similar to the HCF binding domain of the basic leucine-zipper proteins Luman and Zhangfei (ZF). Luman activates promoters containing cAMP or unfolded protein response elements (UPRE). Zhangfei suppresses the transcriptional activity of Luman, but requires HCF binding which may target Luman and Zhangfei to a common location. Sequence analysis predicts that the deduced 272-amino acid Zhangfei protein has a negatively charged N terminus, a leucine zipper of 6 heptad leucine repeats separated by a conserved 6-amino acid spacer, a basic domain, and a bZIP region. It is also presumed that the N-terminal acidic region of Zhangfei is an activation domain.

推荐稀释比 WB:1/500-1/1000;IHC:1/50-1/100

Western blot analysis of CREBZF in mouse brain, PC-3 lysates using CREBZF antibody

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using CREBZF antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

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