H3 Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

推荐稀释比 WB: 1/500-1/1000  IHC: 1/100-1/200  IF: 1/50-1/200  FC: 1/50

Western

Western blot analysis of Phospho-Histone H3 (Thr3) in HeLa lysates treated with FBS + Calyculin A using Phospho-Histone H3 (Thr3) antibody.

Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded human tonsil using Phospho-Histone H3 (T3) antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

-15到-25度