Protocol for labeling epileptic-status-related neuronal ensembles in mouse hippocampal kindling model
Nanxi Lai, Zhisheng Li, Zhong Chen, Yi Wang
Journal：STAR Protocols
IF：0
DOI：10.1016/j.xpro.2023.102255
PMID：
Published：2023-04-25
research field：神经科学动物模型癫痫
Abstract
Summary Epileptic networks are characterized by two states, seizures or more prolonged interictal periods. Here, we present the procedure for labeling seizure-activated and interictal-activated neuronal ensembles in mouse hippocampal kindling model using an enhanced-synaptic-activity-responsive element. We describe the seizure model establishment, tamoxifen induction, electrical stimulation, and calcium signal recording of labeled ensembles. This protocol has demonstrated dissociated calcium activities in the two ensembles during focal seizure dynamics and can be applied to other animal models of epilepsy. For complete details on the use and execution of this protocol, please refer to Lai et al. (2022).1
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