A protocol to characterize zebrafish LGP2 as a dual regulator of IFN response during viral infection
Xiu-Ying Gong, Yi-Bing Zhang
Journal：STAR Protocols
IF：0
DOI：10.1016/j.xpro.2022.101844
PMID：
Published：2022-11-11
research field：分子生物学免疫学遗传学病毒学
Abstract
Summary Here, we present a protocol to characterize zebrafish LGP2 as a dual regulator of interferon (IFN) response. We detail in vivo assays using time-lapse comparison of IFN response between wild-type and lgp2 knockout zebrafish following spring viraemia of carp virus (SVCV) infection. We also describe in vitro assays including titration of infection duration in SVCV-infected fish cells to determine changes in IFN response. This protocol is effective to illuminate a regulatory switch of LGP2 in fish cells toward virus infection. For complete details on the use and execution of this protocol, please refer to Gong et al. (2022).1
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