Abstract

Hydrazine polymers were synthesized under the condition of hydrazine hydrate/ethanol by a clever one-pot method, and then treated with methanesulfonyl chloride and acetyl chloride, respectively, two new quinolones were easily obtained. The basic structure characterization of the prepared quinolone polymers was established based on 1H NMR spectra of aromatic rings and branched chains. In terms of cell activity, MTT assay was used to study the anti-proliferation properties of HCT-116, A549, HepG-2, and McF-7 human cancer cell lines and normal cell lines L02 compared with positive control 5-FU.The distribution and nucleation of polymeric drugs in cells were further observed by means of cell imaging . Conventional Annexin V-FITC kit was subsequently used to detect cell cycle arrest with propidium iodide (PI)and to monitor apoptosis induction with flow cytometry. The results showed that both of the novel quinolone polymers had strong cytotoxic compounds to induce cell cycle stop and trigger apoptosis in the G2/M phase in different test cancer cells. In particular, polymer 1 was more effective.