阻塞性睡眠呼吸暂停通过Hifα/Pparα/NF-κB信号轴影响泪腺功能诱导干眼症

Abstract

Purpose:To determine the effect of obstructive sleep apnea syndrome (OSA) on lacrimal gland function and its mechanism.Methods:Male mice aged seven to eight weeks were housed in cages with cyclic intermittent hypoxia to mimic OSA, and the control group was kept in a normal environment. Slit-lamp observation, fluorescein staining, and corneal sensitivity detection are used to assess cornea changes. Tear secretion was detected by phenol red cotton thread, and the pathological changes of lacrimal gland were observed by hematoxylin and eosin staining, oil red O staining, cholesterol and triglyceride kits, immunofluorescence staining, immunohistochemical staining, real-time polymerase chain reaction, transmission electron microscopy, and Western blot.Results:Studies revealed a decreased tear secretion, corneal epithelial defects and corneal hypersensitivity. Myoepithelial cell damage, abnormal lipid accumulation, reduced cell proliferation, increased apoptosis and inflammatory cell infiltration in the lacrimal gland were also seen.Hifα and NF-κB signaling pathways, moreover, were activated, whilePparαwas downregulated, in the lacrimal glands of OSA mice. Fenofibrate treatment significantly alleviated pathological changes of the lacrimal gland induced by OSA.Conclusion:OSA disturbs theHifα/Pparα/NF-κB signaling axis, which affects lacrimal gland structure and function and induces dry eye.