ER (estrogen receptor 1) a member of the steroid receptor superfamily, contains highly conserved DNA binding (DBD) and ligand binding domains (LBD). Through its estrogen-independent and estrogen-dependent activation domains (AF-1 and AF-2, respectively), ER regulates transcription by recruiting coactivator proteins and interacting with general transcriptional machinery. Phosphorylation provides an important mechanism to regulate ER activity. ER is phosphorylated on multiple sites.

Western

Western blot analysis of ER alpha in (1) MCF-7 lysates; (2)T47D lysates using Estrogen Receptor alpha antibody.

Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded Human cervix carcinoma using ER alpha antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Immunofluorescence

Immunofluorescence analysis of Estrogen Receptor alpha in MCF-7 using ER alpha antibody.