性能参数
蛋白别名(Synonyms) |
TL1A; VEGI-251; TNFSF15; TL1; VEGI; VEGI192A |
种属(Species) |
Human |
表达系统(Source) |
HEK293 |
表达序列(Sequence) |
Asp91-Leu251 |
Accession |
O95150-1 |
标签(Tag) |
N-His-Flag |
分子量(Molecular Weight) |
The protein has a predicted MW of 58.2 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Bis-Tris PAGE result. |
内毒素(Endotoxin) |
Less than 1EU per μg by the LAL method. |
纯度(Purity) |
> 95% as determined by Bis-Tris PAGE; |
活性(Activity) |
ELISA Data: Immobilized Mouse DR3, His Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for Biotinylated Human TNFSF15 Trimer, His Tag with the EC50 of 0.22 μg/ml determined by ELISA . |
制剂(Formulation) |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
外观(Appearance) |
冻干粉 |
储存条件
-25~-15℃保存,收到货之后有效期1年。复溶后, 2 ~8 °C保存,7天有效期。复溶后, -85~-65℃保存,3个月有效期。
复溶方法
开盖之前,先离心。使用蒸馏水复溶冻干的蛋白质,复溶浓度超过100 μg/mL。建议首次使用时将蛋白分装为每次实验用量,避免反复冻融。
注意事项
Ver. CN20240905