Abstract

BackgroundDecidual macrophages maintain immune tolerance and support decidualization at the maternal–fetal interface. However, the heterogeneity and functions of macrophage subsets in recurrent spontaneous abortion (RSA) remain unclear.MethodsWe integrated single-cell RNA sequencing of human endometrium and decidua with clinical validation. Functional assays using THP-1–derived macrophages and the co-culture with decidual stromal cells were performed to characterize macrophage subsets and investigate their roles in decidualization and RSA pathogenesis.ResultsA subset of FOLR2+ macrophages enriched in the decidua showed an M2-like immunoregulatory phenotype. In RSA decidua, FOLR2 expression and the proportion of FOLR2+ macrophages were markedly reduced, accompanied by increased inflammatory signaling and impaired angiogenic and adhesion interactions. Functional assays showed that FOLR2 overexpression enhanced immunoregulatory, tissue-resident, and pro-angiogenic properties, whereas FOLR2 silencing promoted inflammatory responses and impaired angiogenesis. Reciprocal crosstalk between DSCs and FOLR2+ macrophages established a positive feedback loop that promoted stromal decidualization and supported pregnancy maintenance.ConclusionsFOLR2+ macrophages represent an immunoregulatory subset at the maternal–fetal interface with tissue-resident and pro-angiogenic features. The loss of FOLR2+ macrophages in RSA may contribute to immune dysregulation and defective decidualization, providing new insights into the immune mechanisms underlying RSA pathogenesis.